0 avis
Multilocus sequence analysis reveals the genetic diversity of European fruit tree phytoplasmas and supports the existence of inter-species recombination
Archive ouverte
Edité par CCSD ; Microbiology Society -
UMR BGPI Equipe 6; Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699. International audience. The genetic diversity of three temperate fruit tree phytoplasmas ' C andidatus Phytoplasma prunorum', ' C a. P. mali' and ' C a. P. pyri' has been established by multilocus sequence analysis. Among the four genetic loci used, the genes imp and aceF distinguished 30 and 24 genotypes, respectively, and showed the highest variability. Percentage of substitution for imp ranged from 50 to 68 % according to species. Percentage of substitution varied between 9 and 12 % for aceF, whereas it was between 5 and 6 % for pnp and secY. In the case of ' C a P. prunorum' the three most prevalent aceF genotypes were detected in both plants and insect vectors, confirming that the prevalent isolates are propagated by insects. The four isolates known to be hypo-virulent had the same aceF sequence, indicating a possible monophyletic origin. Haplotype network reconstructed by e B URST revealed that among the 34 haplotypes of ' C a. P. prunorum', the four hypo-virulent isolates also grouped together in the same clade. G enotyping of some Spanish and Azerbaijanese ' C a. P. pyri' isolates showed that they shared some alleles with ' C a. P. prunorum', supporting for the first time to our knowledge, the existence of inter-species recombination between these two species
Consulter en ligne
Suggestions
Du même auteur
