Histone deacetylase 3 is required for centromeric H3K4 deacetylation and sister chromatid cohesion

Archive ouverte

Eot-Houllier, Grégory | Fulcrand, Géraldine | Watanabe, Yoshinori | Magnaghi-Jaulin, Laura | Jaulin, Christian

Edité par CCSD ; Cold Spring Harbor Laboratory Press -

International audience. We describe here the role of histone deacetylase 3 (HDAC3) in sister chromatid cohesion and the deacetylation of histone H3 Lys 4 (H3K4) at the centromere. HDAC3 knockdown induced spindle assembly checkpoint activation and sister chromatid dissociation. The depletion of Polo-like kinase 1 (Plk1) or Aurora B restored cohesion in HDAC3-depleted cells. HDAC3 was also required for Shugoshin localization at centromeres. Finally, we show that HDAC3 depletion results in the acetylation of centromeric H3K4, correlated with a loss of dimethylation at the same position. These findings provide a functional link between sister chromatid cohesion and the mitotic “histone code”.

• Description
• Sujet/Public
• Outil
• Outil d'anticipation
• Mémoire et thèse
• Gestion