Exploration of spinal cord injury in a murin model by Broadband Coherent Anti-Stokes Raman Scattering (B-CARS) Microscopy

Archive ouverte

Cloitre, Thierry | de Lizaraga, Joshua | Manesco, Clara | Gerber, Yannick Nicolas | Perrin, Florence Evelyne | Camp, Charles | Gergely, Csilla

Edité par CCSD -

International audience. Broadband CARS imaging is used to evaluate changes underlying the lesion of a spinal cord injury in a murine model. After injury the axonal regeneration in spinal cord is partly inhibited by the glial scar, acting as a physical and chemical barrier. Recently we have reported that the SHG signal exhibited by fibrillar collagen enabled to specifically monitor it as a biomarker of a spinal cord lesion1. However, the injury and the scarring process involves also other elements as the myelin sheets, microglia, astrocytes and extracellular matrix components. We assess the additional structural and metabolic modifications of the injured tissue via BCARS imaging of excised murine spinal cord tissue at different time points (72h, 1, 2, 6 weeks) after injury. BCARS is based on the detection of the fingerprint region of molecules (500-1500 cm-1) and could probe multiple Raman transitions simultaneously, to allow a “chemical” imaging of biological tissues with improved molecular contrast2. When compared to spontaneous Raman microscopy, BCARS microscopy provided 10-100x faster image acquisition for quantitative and qualitative assessment of pharmaceuticals at much higher spatio-chemical resolution and with spectra of much higher signal-to noise ratio.

• Description
• Sujet/Public
• Outil
• Outil d'anticipation
• Mémoire et thèse
• Gestion