Label-Free imaging by Broadband Coherent Anti-Stokes Raman Scattering (B-CARS) Microscopy

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Cloitre, Thierry | Camp, Charles | Gergely, Csilla

Edité par CCSD -

International audience. Broadband CARS imaging is one of the Coherent Raman imaging (CRI) modalities with sub-cellular spatial resolution and providing an intrinsic fingerprint of chemical composition1. For biological samples, most of the characteristic Raman peaks are found in the ‘fingerprint’ spectral region, (~500 cm−1 to 1,800 cm−1 ), with the remaining found in the higher-energy CH-/OH-stretch region (~2,700 cm−1 to 3,300 cm−1). The various CRI techniques exhibit a large range in both performance and experimental setup complexity. For example, narrowband CARS microscopes are commercially available and could acquire image pixels very quickly but only probes narrow regions of the Raman spectrum. On the other hand, B-CARS acquire the full spectrum at the cost of longer pixel dwell time. In this talk, we will present the principles of the B-CARS microscopy we’ve developed in our laboratory and some examples of biological applications.

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