Bulges in left-handed G-quadruplexes

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Das, Poulomi | Ngo, Khac Huy | Winnerdy, Fernaldo richtia | Maity, Arijit | Bakalar, Blaž | Mechulam, Yves | Schmitt, Emmanuelle | Phan, Anh Tuân

Edité par CCSD ; Oxford University Press -

International audience. G-quadruplex (G4) DNA structures with a left-handed backbone progression have unique and conserved structural features. Studies on sequence dependency of the structures revealed the prerequisites and some minimal motifs required for left-handed G4 formation. To extend the boundaries, we explore the adaptability of left-handed G4s towards the existence of bulges. Here we present two X-ray crystal structures and an NMR solution structure of left-handed G4s accommodating one, two and three bulges. Bulges in left-handed G4s show distinct characteristics as compared to those in right-handed G4s. The elucidation of intricate structural details will help in understanding the possible roles and limitations of these unique structures.

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