Bisphenol S is present in culture media used for ART and cell culture

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Togola, A | Desmarchais, A | Téteau, O | Vignault, C | Maillard, Virginie | Buron, C | Bristeau, S | Guerif, Fabrice | Binet, A | Elis, Sébastien

Edité par CCSD ; Oxford University Press (OUP) -

International audience. STUDY QUESTION: Do plastic laboratory consumables and cell culture media used in ART contain bisphenols?SUMMARY ANSWER: The majority of human embryo culture media assessed contained bisphenol S close to the nanomolarconcentration range, while no release of bisphenols by plastic consumables was detected under routine conditions.WHAT IS KNOWN ALREADY: The deleterious effect of the endocrine disruptor bisphenol A (BPA) on female fertility raised concernsregarding ART outcome. BPA was detected neither in media nor in the majority of plastic consumables used in ART; however, it mighthave already been replaced by its structural analogs, including bisphenol S (BPS).STUDY DESIGN, SIZE, DURATION: Seventeen plastic consumables and 18 cell culture and ART media were assessed for thepresence of bisphenols.PARTICIPANTS/MATERIALS, SETTING, METHODS: Ten different bisphenols (bisphenol A, S, AF, AP, B, C, E, F, P and Z) weremeasured using an isotopic dilution according to an on-line solid phase extraction/liquid chromatography/mass spectrometry method.MAIN RESULTS AND THE ROLE OF CHANCE: While the plastic consumables did not release bisphenols under routine conditions,16 of the 18 cell culture and ART media assessed contained BPS. Six media exhibited BPS concentrations higher than 1 nM and reached upto 6.7 nM (1693 ng/l).LARGE SCALE DATA: N/A.LIMITATIONS, REASONS FOR CAUTION: Further studies are required to investigate a greater number of ART media to identifyless potentially harmful ones, in terms of bisphenol content.WIDER IMPLICATIONS OF THE FINDINGS: As BPS has already been reported to impair oocyte quality at nanomolar concentrations,its presence in ART media, at a similar concentration range, could contribute to a decrease in the ART success rate. Thus far, therehas been no regulation of these compounds in the ART context.STUDY FUNDING/COMPETING INTERESTS: This study was financially supported by the ‘Centre-Val de Loire’ Region (Bemolproject, APR IR 2017), INRAE, BRGM, the French National Research Agency (project ANR-18-CE34-0011-01 MAMBO) and theBioMedicine Agency (Project 18AMP006 FertiPhenol). The authors declare that they have no conflict of interest that could be perceived asprejudicing the impartiality of the reported research.

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