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LUEGO: a cost and time saving gel shift procedure.

Archive ouverte | Jullien, Nicolas | CCSD

International audience. We developed a new approach to prepare DNA probes for electrophoretic mobility gel shift assays that presents a number of advantages compared with the classical approach. The method relies on...

Regulation of Cre recombinase by ligand-induced complementation of inactive fragments

Archive ouverte | Jullien, Nicolas | CCSD

International audience. Cre recombinase is extensively used to engineer the genome of experimental animals. However, its usefulness is still limited by the lack of an ef®cient temporal control over its activity. To ...

Conditional genome engineering in Toxoplasma gondii uncovers alternative invasion mechanisms

Archive ouverte | Andenmatten, Nicole | CCSD

International audience. We established a conditional site–specific recombination system based on dimerizable Cre– mediated recombination in the apicomplexan parasite Toxoplasma gondii. Using a novel single vector st...

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