Conditional genome engineering in Toxoplasma gondii uncovers alternative invasion mechanisms

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Andenmatten, Nicole | Egarter, Saskia | Jackson, Allison, J | Jullien, Nicolas | Herman, Jean-Paul | Meissner, Markus

Edité par CCSD ; Nature Publishing Group -

International audience. We established a conditional site–specific recombination system based on dimerizable Cre– mediated recombination in the apicomplexan parasite Toxoplasma gondii. Using a novel single vector strategy that allows ligand-dependent, efficient removal of a gene of interest, we generated three knockouts of apicomplexan genes considered essential for host-cell invasion. Our findings uncover the existence of an alternative invasion pathway in apicomplexan parasites.

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