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Harnessing MalE for the study of antigen/antibody recognitions

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Bedouelle, Hugues | Renard, Martial | Belkadi, Laurent | England, Patrick

Edité par CCSD ; Elsevier -

International audience. The construction of hybrids between the variable fragment (Fv) of antibodies and protein MalE of Escherichia coli at the genetic level makes possible their preparation in a functional state, independently of any interaction with the antigen. We used such hybrids and a mutagenesis approach to study the recognition between antibody D1.3 and its antigen lysozyme, and its maturation. We subsequently transformed D1.3 into a reagentless fluorescent biosensor by knowledge-based design.

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