Rouxiella chamberiensis gen. nov., sp. nov., a new Enterobacteriaceae isolated from parenteral nutrition bags.

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Le Fleche-Mateos, Anne | Levast, Marion | Lomprez, Fabienne | Arnoux, Yolande | Andonian, Clément | Perraud, Michel | Vincent, Véronique | Ar Gouilh, Meriadeg | Thiberge, Jean-Michel | Vandenbogaert, Mathias | Diancourt, Laure | Caro, Valérie | Burguiere, Ana Maria | Manuguerra, Jean-Claude

Edité par CCSD ; Microbiology Society -

International audience. Parenteral nutrition bags for newborns were found contaminated by a previously undescribed 23 Enterobacteriaceae. The six isolates studied by rrs - (encoding 16S rRNA) and multilocus 24 sequence analysis (MLSA) formed a discrete branch close to genera Ewingella, Rahnella, 25 Yersinia, Hafnia and Serratia. Phenotypically, the new taxon was distinct from these four 26 genera. The new taxon gave positive Voges-Proskauer, Simmons citrate, o-nitrophenyl-β-27 galactoside hydrolysis tests; fermented D-glucose, D-mannitol, L-rhamnose, D-melibiose, L-28 arabinose, D-xylose, and hydrolyzed esculin; did not ferment maltose, trehalose, raffinose, D-29 sorbitol, sucrose and D-cellobiose. The following tests, motility, gas production, urease, 30 gelatinase, and nitrate reduction were also negative. All isolates failed to grow at 37°C. 31 Therefore, the new taxon is proposed as a new species and genus and named Rouxiella 32 chamberiensis gen. nov., sp. nov. The type strain is 130333T (= CIP 110714T = DSM 28324T). 33 The G+C content of the type strain DNA was 53 mol%.

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