Afficher la couverture 'Gene targeting in rats using transcription activator-like effector nucleases | Ménoret, Séverine' en grand format
/5

0 avis

Gene targeting in rats using transcription activator-like effector nucleases

Archive ouverte

Ménoret, Séverine | Tesson, Laurent | Rémy, Severine | Usal, Claire | Thépenier, Virginie | Thinard, Reynald | Ouisse, Laure-Hélène | de Cian, Anne | Giovannangeli, Carine | Concordet, Jean-Paul | Anegon, Ignacio

Edité par CCSD ; Elsevier -

International audience. The rat is a model of choice to understanding gene function and modeling human diseases. Since recent years, successful engineering technologies using gene-specific nucleases have been developed to gene edit the genome of different species, including the rat. This development has become important for the creation of new rat animals models of human diseases, analyze the role of genes and express recom-binant proteins. Transcription activator-like (TALE) nucleases are designed nucleases consist of a DNA binding domain fused to a nuclease domain capable of cleaving the targeted DNA. We describe a detailed protocol for generating knockout rats via microinjection of TALE nucleases into fertilized eggs. This technology is an efficient, cost-and time-effective method for creating new rat models.

Suggestions

Du même auteur

Efficient gene targeting by homology-directed repair in rat zygotes using TALE nucleases

Archive ouverte | Remy, Séverine | CCSD

International audience. The generation of genetically modified animals is important for both research and commercial purposes. The rat is an important model organism that until recently lacked efficient genetic engi...

Genome Editing in Rats Using TALE Nucleases

Archive ouverte | Tesson, Laurent | CCSD

International audience. The rat is an important animal model to understand gene function and model human diseases. Since recent years, the development of gene-specific nucleases has become important for generating n...

Homology-directed repair in rodent zygotes using Cas9 and TALEN engineered proteins

Archive ouverte | Ménoret, Séverine | CCSD

International audience. The generation of genetically-modified organisms has been revolutionized by the development of new genome editing technologies based on the use of gene-specific nucleases, such as meganucleas...

Chargement des enrichissements...