Genome Editing in Rats Using TALE Nucleases

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Tesson, Laurent | Remy, Severine | Ménoret, Séverine | Usal, Claire | Thinard, Reynald | Savignard, Chloé | de Cian, Anne | Giovannangeli, Carine | Concordet, Jean-Paul | Anegon, Ignacio

Edité par CCSD -

International audience. The rat is an important animal model to understand gene function and model human diseases. Since recent years, the development of gene-specific nucleases has become important for generating new rat models of human diseases, to analyze the role of genes and to generate human antibodies. Transcription activator-like (TALE) nucleases efficiently create gene-specific knockout rats and lead to the possibility of gene targeting by homology-directed recombination (HDR) and generating knock-in rats. We describe a detailed protocol for generating knockout and knock-in rats via microinjection of TALE nucleases into fertilized eggs. This technology is an efficient, cost- and time-effective method for creating new rat models.

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