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Integration of bioguided fractionation and molecular networking to identify anti-mycotoxins from grapevine
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International audience. According to Food and Agricultural Organization, 25% of the world’s crops are affected by mycotoxins each year. In Europe, cereals are the most impacted crops, mainly by Fusarium graminearum which produces type B trichothecenes (TCTB). Deoxynivalenol, the predominant TCTB and most common contaminant of cereals, is responsible for digestive disorders such as vomiting due to its strong emetic effects after consumption, because it is transported into the brain, where it runs dopaminergic receptors. In order to substitute synthetic fungicides and durably control toxigenic fungal infections, we aimed to exploit the biological activity of phenolic compounds from grapevine by-products. In this study, an extract of grapevine canes was investigated for its inhibitory potential towards F. graminearum mycotoxin production. A bioassay-guided fractionation of this extract using centrifugal partition chromatography was performed together with a UPLC-MSn-based metabolic profiling of raw extract and each fraction obtained. In vitro assays (TCTB quantification in fungal culture media) and dereplication with molecular networking revealed that fractions containing stilbenoids are particularly effective. These results show that grapevine canes are a promising feedstock of candidates able to control F. graminearum mycotoxin production.
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