0 avis
Natural compounds from vine by-products as promising antifungal and antimycotoxin drugs
Archive ouverte
Edité par CCSD -
International audience. Driven by global warming, contamination of agricultural commodities with mycotoxins (e.g., deoxynivalenol (DON), fumonisins (FB), T-2 toxin, zearalenone, aflatoxins (AF), ochratoxins and patulin) becomes an intractable problem worldwide, threatening global food security. Searching for eco-friendly antimycotoxin drugs is an urgent need to lower the dependence on agrochemicals. This current study thus aims at proving the antifungal and antimycotoxin activity of main active biomolecules derived from vine by-product extracts via treatments with Fusarium graminearum fungus - the main producer of type B trichothecene mycotoxins (TCTB) on wheat in Europe. Via biofractionational strategies and high-resolution LC-MS/MS analyses, these active molecules were structurally identified as phenolic compounds with various degrees of polymerization. Their biological effects on spore germination, biomass production, and TCTB biosynthesis by F. graminearum were then evaluated using in vitro assays. Data illustrated that these effects of oligomeric compounds were higher than those of monomeric ones. A stronger antimycotoxin activity was furthermore found in methylated compounds when compared to their non-methylated forms. Remarkably, the TCTB biosynthesis by F. graminearum was significantly inhibited (higher than 60%) by the active molecules at very low concentrations, between 10 and 40 µM. These findings hold promise for the development of new antifungal and antimycotoxin solutions exploiting viticulture wastes to minimize the contamination of cereals with mycotoxins. Further transcriptomics and metabolomics analyses will provide us with deeper insights into their molecular modes of action on the antimycotoxin activity.
Consulter en ligne
Suggestions
Du même auteur
