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eIF4E1 precision editing provides a solution to the trade-off between the plant development and potyvirusresistance albeit with a low durability
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International audience. components of the plant cell machinery. For example, in Tomato, resistance to the PVY N605 isolate can be achieved by knocking out both eIF4E1 and eIF4E2 genes, but is associated with profound effect on the plant development (Gauffier et al. Plant J., 2016). A solution is to design functional alleles encoding host proteins which retain their initial role in the plant physiology but that the virus cannot recruit any longer. Recently, we showed as a proof of concept the conception of an eIF4E1 resistance allele in cherry tomato obtained by genome editing based on the knowledge resulting from the study of natural functional alleles in crops (Kuroiwa et al., PBJ, 2024). Here, we further assess this allele for its effect on plant development and resistance durability.We launched a whole-plant phenotyping on a set of tomato genotypes where the endogenous eIF4E1gene isedited in the two regions acknowledged to be involved in resistance to potyviruses. We show that the eIF4E1-editing in tomatoes do not affect the plant growth and development in a greenhouse setting. We proceeded with the evaluation of the virus resistance durability. We show that most resistant edited SleIF4E1 suppresses the PVY N605 viral accumulation to a significantly lower level than the wild-type SleIF4E1 but that this resistance can be broken down substantially whithin nine weeks after inoculation. This is confirmed by the virus obtaining signature mutations of resistance breaking.Altogether, this work assesses the efficacy of resistance breeding strategy by the base-editing in tomato SleIF4E1 in an applicational point of view, but calls for further improvement in the resistance allele design and obtention.
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