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Attempts at translating resistance to potexvirus from Arabidopsis to tomato by inducing loss-of-susceptibility
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International audience. A major goal of plant breeding is to translate genetic resistances to viruses across species and in the geneticbackground of choice. In the case of susceptibility factors, resistance to viruses can be achieved by genome editing following the inactivation (knock-out) or modification of Susceptibility (S) genes, but how these mechanisms can be translated from model plant to crops is not always clear. Moreover, for breeding purposes, resistance should not be developed at the expense of the plant development. Here, we focus on two S genes involved in resistance to potexvirus, namely nCBP (novel Cap Binding Protein), encoding a protein related to the family of eIF4E translation initiation factors, and EXA1 (Essentials for poteXvirus Accumulations 1), which encodes a GYF domain-containing protein.It has been previously shown that i) inactivating nCBP or EXA1 in Arabidopsis limit the viral propagation of thepotexviruses PlAMV (Hashimoto et al. 2016, Plant J) and that ii) EXA1 and eIF4E family members play additiveroles on the accumulation of Potexvirus (Nishikawa et al. 2023 J Virol.). Here, to translate those results to crop, we describe the production of ncbp and exa1 mutants in different tomato genetic backgrounds, using genome editing techniques. We will present preliminary results on resistance to viruses as well as effect of the mutations on the plant and fruit development. This might provide useful insight on the transferability of those S genes for breeding purposes, as well as information on the trade-off between resistance and development.
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