Correlation between Luminex donor‐specific crossmatches and levels of donor‐specific antibodies in pretransplantation screening

Archive ouverte

Guillaume, N. | Mazouz, H. | Piot, V. | Presne, C. | Westeel, P.‐f.

Edité par CCSD ; Wiley -

International audience. Before kidney transplantation, recipients are routinely screened for preformed antibodies and prospective crossmatches. In this study, we compared prospective Luminex donor‐specific crossmatches ( LumXm ) with the levels of identified, donor‐specific antibodies ( DSAs ). LumXm was performed for 108 patient sera, 84 of which were positive for preformed antibodies and 24 of which were negative. Although LumXm can detect class I DSAs (anti‐A and anti‐B) with a mean fluorescence intensity ( MFI ) as low as 2300, the assay has a ‘grey zone’ for MFIs up to 4000 with a sensitivity of 54% and a specificity of 100%. LumXm can detect a class II DSA (anti‐ DRB1 ) with an MFI as low as 1300 and a sensitivity of 93% and a specificity of 99%. However, these correlations were obtained with two precautions: autocrossmatching and single‐antigen bead assay with denaturing buffer were performed in discordant cases. Moreover, LumXm failed to detect anti‐Cw and anti‐ DP in the 10 cases studied. LumXm , therefore, displays certain discrepancies with respect to single‐bead assays – especially for antibodies with a low MFI . Unfortunately, LumXm has a low sensitivity for anti‐A and anti‐B class I antibodies.

• Description
• Sujet/Public
• Outil
• Outil d'anticipation
• Mémoire et thèse
• Gestion