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Mini-FLOTAC for counting Toxoplasma gondii oocysts from cat feces – Comparison with cell counting plates
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International audience. Oocysts of Toxoplasma gondii represent one of the most common environmental contaminants causingthe zoonotic infection toxoplasmosis. The aim of the present study was to compare the Mini-FLOTAC devicewith traditional cell counting plates (Kova Slide) for the detection of T. gondii oocysts from feline feces.Two types of experiments were performed: (i) purified oocysts were counted in different dilutions and(ii) specific pathogen free T. gondii-negative cat feces was inoculated with numbers of purified oocystsand counting was performed directly from feces. Our analysis showed a thousand times higher sensitivityof Mini-FLOTAC (5 × 102 oocysts) compared to Kova Slide (5 × 105 oocysts). Also, when comparedby McNemar’s test, counting of the purified oocysts showed a higher sensitivity of Mini-FLOTAC comparedto Kova Slide, for a dilution of 103 oocysts/ml (chi2 = 6.1; P < 0.05). A better sensitivity was also foundwith Mini-FLOTAC in dilutions of 105 and 104 oocysts/ml, when counted from feces (chi2 = 4.2 and 8.1,respectively, P < 0.05). Our results show that Mini-FLOTAC is more sensitive than traditional methods ofT. gondii oocysts detection and quantification is more accurate. Furthermore, Mini-FLOTAC simplicity andcost effectiveness allow it to be used with light microscopes in any laboratory or field conditions. Wetherefore recommend its use for regular screening. Further studies are needed to validate Mini-FLOTACfor the detection of oocysts in soil and water samples in field conditions.
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