De novo sequencing and assembly of genomes using long reads

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Aury, Jean-Marc

Edité par CCSD -

International audience. The introduction of short-reads technologies ten years ago, significantly increased the number of available genomes. Generally, these assemblies are incomplete and fragmented, and only a few of them are at the chromosome-scale. Recently, Oxford Nanopore sequencing technology was commercialized with the promise to sequence long DNA fragments (kilobases to megabases order) and then, by using efficient algorithms, provide assembly of high quality in terms of contiguity and completeness of the repetitive regions. This presentation will provide an overview of the capability of current technologies to generate chromosome-scale assemblies and highlight some recent projects of plant and animal genomes.

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