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De novo sequencing and assembly of complex genomes

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Aury, Jean-Marc

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International audience. Reconstructing plant genomes is a difficult task due to their often large sizes, unusual ploidy and large numbers of repeated elements. However, the field of sequencing is changing very rapidly, with new and improved methods released every year. With the rise of long-read and long-range technologies, the delivery of chromosome-scale assemblies is now possible for almost all species. This presentation will show the advanced techniques already existing in the field and illustrate their application to generate high-quality assemblies.

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