Protocol to image and analyze hippocampal network dynamics in non-anesthetized mouse pups

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Protocol to image and analyze hippocampal network dynamics in non-anesthetized mouse pups

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Ratsifandrihamanana, Mirindra, R | Dard, Robin, F | Denis, Julien | Cossart, Rosa | Picardo, Michel, A

International audience. Two-photon calcium imaging is a powerful technique that has revolutionized our understanding of how neural circuit dynamics supports different behaviors and cognitive processes. However, performing imaging during development remains challenging. Here, we provide a protocol to image CA1 neurons in mouse pups as well as a pipeline of analysis to analyze and share the data. We describe steps for intracerebroventricular injection, cranial window surgery, two-photon calcium imaging, and analysis of imaging data.

Langue: en

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