Generation of a GPR146 knockout human induced pluripotent stem cell line (ITXi001-A-1)

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Bray, Lise | Caillaud, Amandine | Girardeau, Aurore | Patitucci, Murielle | Le May, Cédric | Cariou, Bertrand | Rimbert, Antoine

Edité par CCSD ; Elsevier -

International audience. Dyslipidemia is a key modifiable causal risk factor involved in the development of atherosclerotic cardiovascular disease. Recently, the G protein-coupled receptor 146 (GPR146), a member of the G-coupled protein receptors' family, has been shown to be a regulator of plasma cholesterol. Inhibition of hepatic GPR146 in mice displays protective effect against both hypercholesterolemia and atherosclerosis. Here, we characterize a genetically engineered human induced pluripotent stem cell (hiPSC) model invalidated for GPR146 (ITXi001-A-1) using CRISPR-Cas9 editing technology. Differentiation of ITXi001-A-1 towards hepatic fate will provide a suitable model for deciphering the molecular mechanisms sustaining the beneficial metabolic effects of GPR146 inhibition.

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