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Twenty-two years later: Consistent dose estimation of an accidental overexposure by retrospective biological dosimetry
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International audience. As the chromosomal translocation rate increases with age in the non-exposed population, the translocation-based dose estimation of an external radiation exposure victim needs to take into consideration such background. We sought to retrospectively estimate the dose of exposure of a victim from the Lilo radiological accident in Georgia twenty-two years afterwards and compare it to the original biological dosimetry-based dose calculation performed in our laboratory. Similar types of studies have retrospectively estimated a radiation dose, notably involving victims of the Chernobyl, Goiânia and Tammiku accidents [1][2][3]. Nevertheless, their estimations were done after shorter periods of time post-exposure and in some cases, the exposure might not have been exclusively of an external nature [1][2].In this study, we used Fluorescence In Situ Hybridization (FISH) to detect and score chromosomal translocations in lymphocytes from a recent blood sample of the victim. We performed the analysis using our laboratory’s updated FISH dose-effect curve and taking into account translocation data from a large panel of unexposed individuals. We found the mean exposure dose to be similar to the original assessment obtained by the dicentric chromosome assay (DCA) more than 22 years ago. Furthermore, the confidence interval from the DCA analysis was contained within our FISH confidence interval, which as expected, was slightly larger. Altogether these observations confirm a comparable dose estimation.In conclusion, retrospective biological dosimetry by FISH allowed us to estimate a dose that is consistent with the original assessment 22 years prior. This suggests that our current dose-effect curve could be used for relative dose estimations long time after external exposure.
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