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Comparison of the efficiency of different newcastle disease virus reverse genetics systems

Archive ouverte | Harada, Liu-Ji | CCSD

International audience. Rescue of negative-sense single-stranded RNA viruses ((-)ssRNA virus), generally requires-the handling of a large number of plasmids to provide the virus genome and essential components for g...

Two-plasmid system to increase the rescue efficiency of paramyxoviruses by reverse genetics: The example of rescuing Newcastle Disease Virus

Archive ouverte | Harada, Liu-Ji | CCSD

Within paramyxoviruses, conventional reverse genetics require the transfection of a minimum of four plasmids: three to reconstruct the viral polymerase complex that replicates and expresses the virus genome delivered by a fourth p...

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