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Single-molecule kinetic locking allows fluorescence-free quantification of protein/nucleic-acid binding

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Rieu, Martin | Valle-Orero, Jessica | Ducos, Bertrand | Allemand, Jean-François | Croquette, Vincent

Edité par CCSD ; Nature Publishing Group -

International audience. Fluorescence-free micro-manipulation of nucleic acids (NA) allows the functional characterization of DNA/RNA processing proteins, without the interference of labels, but currently fails to detect and quantify their binding. To overcome this limitation, we developed a method based on single-molecule force spectroscopy, called kinetic locking , that allows a direct in vitro visualization of protein binding while avoiding any kind of chemical disturbance of the protein’s natural function. We validate kinetic locking by measuring accurately the hybridization energy of ultrashort nucleotides (5, 6, 7 bases) and use it to measure the dynamical interactions of Escherichia coli/E. coli RecQ helicase with its DNA substrate.

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