Beneficial protective role of endogenous lactic acid bacteria against mycotic contamination of honeybee beebread

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Janashia, Irakli | Choiset, Yvan | Jozefiak, Damian | Déniel, Franck | Coton, Emmanuel | Moosavi-Movahedi, A.A | Chanishvili, Nina | Haertlé, Thomas

Edité par CCSD -

International audience. The purpose of this article is to reveal the role of the lactic acid bacteria (LAB) in the beebread transformation/preservation, biochemical properties of 25 honeybee endogenous Lstrains, particularly: antifungal, proteolytic, and amylolytic activities putatively expressed in the beebread environment have been studied. Seventeen fungal strains isolated from beebread samples were identified and checked for their ability to grow on simulated beebread substrate (SBS) and then used to study mycotic propagation in the presence of LAB. Fungal strains identified as Aspergillus niger (Po1), Candida sp. (BB01), and Z. rouxii (BB02) were able to grow on SBS. Their growth was partly inhibited when co-cultured with the endogenous honeybee Lstrains studied. No proteolytic or amylolytic activities of the studied Lwere detected using pollen, casein starch based media as substrates. These findings suggest that some honeybee Lsymbionts are involved in maintaining a safe microbiological state in the host honeybee colonies by inhibiting beebread mycotic contaminations, starch, and protein predigestion in beebread by Lis less probable. Honeybee endogenous Luse pollen as a growth substrate and in the same time restricts fungal propagation, thus showing host beneficial action preserving larval food. This study also can have an impact on development of novel methods of pollen preservation and its processing as a food ingredient.

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