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Protection of honeybee apis mellifera by its endogenous and exogenous lactic flora against bacterial infections
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lactic acid bacteria (lab) symbionts of honeybees are certainly playing key roles in theirhost's colony functioning. the defense against bacterial pathogen invasion by endogenouslab has been considered as promising and usable phenomenon. this study addressesbacteriocinogeny as one of antibacterial action mode displayed by bacteria. the honeybeeendogenous lab isolated from worker honeybee intestines (61 strains), queen honeybeeintestines (16 strains) and beebread (25 strains) were tested for bacteriocin productionability. we checked also well characterized bacteriocin producing lab strains againstbacteria causing american foulbrood (afb) e paenibacillus larvae aiming possible use ofexogenous lab for control afb in honeybees and in the same time to observe thevulnerability of endogenous bacteria exposed to bacteriocin producers. we demonstratedthat none of 102 studied lab strains, isolated from worker honeybee intestines, fromqueen honeybee intestines and from beebread, produced bacteriocins detectable by thewell diffusion method (wdm). all of them failed to inhibit two strains of p. larvae.three exogenous bacteriocin-producing lab strains were tested against the samepathogens and against 25 endogenous bacterial isolates representing 11 different labspecies. the screening showed that all the tested exogenous bacteriocin-producing strainsinhibited the tested p. larvae strains. the endogenous lab strains exhibited varied sensitivityprofiles when treated with bacteriocin-producing strains. this raises similar challengesto those observed in antibiotic applications leading to dysbacteriosis, even thoughthe efficacy of these bacteriocins against p. larvae in an in vitro system is evident.
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