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A complete collection of single‐gene deletion mutants of Acinetobacter baylyi ADP1
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Edité par CCSD ; EMBO Press -
A utilisé MicroScope Platform. International audience. We have constructed a collection of single-gene deletion mutants for all dispensable genes of the soilbacterium Acinetobacter baylyi ADP1. A total of 2594 deletion mutants were obtained, whereas 499(16%) were not, and are therefore candidate essential genes for life on minimal medium. Thisessentiality data set is 88% consistent with the Escherichia coli data set inferred from the Keiomutant collection profiled for growth on minimal medium, while 80% of the orthologous genesdescribed as essential in Pseudomonas aeruginosa are also essential in ADP1. Several strategies wereundertaken to investigate ADP1 metabolism by (1) searching for discrepancies between ouressentiality data and current metabolic knowledge, (2) comparing this essentiality data set to thosefrom other organisms, (3) systematic phenotyping of the mutant collection on a variety of carbonsources (quinate, 2-3 butanediol, glucose, etc.). This collection provides a newresource for the studyof gene function by forward and reverse genetic approaches and constitutes a robust experimentaldata source for systems biology approaches.
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