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In vivo selection of lethal mutations reveals two functional domains in arginyl–tRNA synthetase

Archive ouverte | Geslain, Renaud | CCSD

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In vivo selection of lethal mutations reveals two functional domains in arginyl–tRNA synthetase

Archive ouverte | Geslain, Renaud | CCSD

Using random mutagenesis and a genetic screening in yeast, we isolated 26 mutations that inactivate Saccharomyces cerevisiae arginyl–tRNA synthetase (ArgRS). The mutations were identified and the kinetic parameters of the correspo...

The class II aminoacyl-tRNA synthetases and their active site: Evolutionary conservation of an ATP binding site

Archive ouverte | Eriani, Gilbert | CCSD

International audience

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