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Sequencing and Analysis of Wheat Chromosome 1B

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Choulet, Frédéric | Rimbert, Hélène | Josselin, Ambre-Aurore | Darrier, Benoît | Sourdille, Pierre | Balfourier, François | Barbe, Valérie | Alberti, Adriana, A. | Labadie, Karine | Mangenot, Sophie | Couloux, Arnaud | Wincker, Patrick | Berges, Helene | Alaux, Michael | Letellier, Thomas | Quesneville, Hadi | Frenkel, Zeev | Fahima, Tzion | Korol, Abraham | Paux, Etienne

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We are currently producing a reference sequence of the bread wheat chromosome 1B (535 Mb) by sequencing 6023 BAC clones comprising the minimal tilling path of the physical maps of both short and long arms. BACs were sequenced by pools using a combination of Illumina MiSeq paired-end reads and HiSeq2500 5 kb mate pairs. First round of assembly yielded 7131 scaffolds representing 534 Mb. Report on the improvement of this assembly, the construction of a pseudomolecule and the comparison with the previous sequencing and assembly of chromosome 3B will be presented.

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