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Quantitative Analysis of Intra-chromosomal Contacts: The 3C-qPCR Method

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Ea, V. | Court, F. | Forne, T.

Edité par CCSD ; Humana Press/Springer Imprint -

International audience. The chromosome conformation capture (3C) technique is fundamental to many population-based methods investigating chromatin dynamics and organization in eukaryotes. Here, we provide a modified quantitative 3C (3C-qPCR) protocol for improved quantitative analyses of intra-chromosomal contacts. We also describe an algorithm for data normalization which allows more accurate comparisons between contact profiles.

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