Different effects of the TAR structure on HIV-1 and HIV-2 genomic RNA translation.

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Soto-Rifo, Ricardo | Limousin, Taran | Rubilar, Paulina S | Ricci, Emiliano P | Décimo, Didier | Moncorgé, Olivier | Trabaud, Mary-Anne | André, Patrice | Cimarelli, Andrea | Ohlmann, Théophile

Edité par CCSD ; Oxford University Press -

International audience. The 5'-untranslated region (5'-UTR) of the genomic RNA of human immunodeficiency viruses type-1 (HIV-1) and type-2 (HIV-2) is composed of highly structured RNA motifs essential for viral replication that are expected to interfere with Gag and Gag-Pol translation. Here, we have analyzed and compared the properties by which the viral 5'-UTR drives translation from the genomic RNA of both human immunodeficiency viruses. Our results showed that translation from the HIV-2 gRNA was very poor compared to that of HIV-1. This was rather due to the intrinsic structural motifs in their respective 5'-UTR without involvement of any viral protein. Further investigation pointed to a different role of TAR RNA, which was much inhibitory for HIV-2 translation. Altogether, these data highlight important structural and functional differences between these two human pathogens.

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