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Detection, characterization, and phylogenetic analysis of novel astroviruses from endemic Malagasy fruit bats
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Edité par CCSD ; BioMed Central -
Les affiliations de JM Heraud ont été corrigées dans la correction : DOI 10.1186/s12985-024-02527-3. Le PDF de la correction est en fichier annexe. International audience. Bats (order: Chiroptera) are known to host a diverse range of viruses, some of which present a human publichealth risk. Thorough viral surveillance is therefore essential to predict and potentially mitigate zoonotic spillover.Astroviruses (family: Astroviridae) are an understudied group of viruses with a growing amount of indirect evidencefor zoonotic transfer. Astroviruses have been detected in bats with significant prevalence and diversity, suggestingthat bats may act as important astrovirus hosts. Most astrovirus surveillance in wild bat hosts has, to date, beenrestricted to single-gene PCR detection and concomitant Sanger sequencing; additionally, many bat species andmany geographic regions have not yet been surveyed for astroviruses at all. Here, we use metagenomic NextGeneration Sequencing (mNGS) to detect astroviruses in three species of Madagascar fruit bats, Eidolon dupreanum,Pteropus rufus, and Rousettus madagascariensis. We detect numerous partial sequences from all three speciesand one near-full length astrovirus sequence from Rousettus madagascariensis, which we use to characterize theevolutionary history of astroviruses both within bats and the broader mammalian clade, Mamastrovirus. Takentogether, applications of mNGS implicate bats as important astrovirus hosts and demonstrate novel patterns of batastrovirus evolutionary history, particularly in the Southwest Indian Ocean region.
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