Afficher la couverture 'The gut environment regulates bacterial gene expression which modulates susceptibility to bacteriophage infection | Lourenço, Marta' en grand format
/5

0 avis

The gut environment regulates bacterial gene expression which modulates susceptibility to bacteriophage infection

Archive ouverte

Lourenço, Marta | Chaffringeon, Lorenzo | Lamy-Besnier, Quentin | Titécat, Marie | Pédron, Thierry | Sismeiro, Odile | Legendre, Rachel | Varet, Hugo | Coppée, Jean-Yves, J.-Y. | Bérard, Marion | de Sordi, Luisa | Debarbieux, Laurent

Edité par CCSD -

International audience. Abundance and diversity of bacteria and their viral predators, bacteriophages (phages), in the digestive tract are associated with human health. Particularly intriguing is the long-term coexistence of these two antagonistic populations. We performed genome-wide RNA sequencing on a human enteroaggregative Escherichia coli isolate to identify genes differentially expressed between in vitro conditions and in murine intestines. We experimentally demonstrated that four of these differentially expressed genes modified the interactions between E. coli and three virulent phages by either increasing or decreasing its susceptibility/resistance pattern and also by interfering with biofilm formation. Therefore, the regulation of bacterial genes expression during the colonization of the digestive tract influences the coexistence of phages and bacteria, highlighting the intricacy of tripartite relationships between phages, bacteria, and the animal host in intestinal homeostasis.

Suggestions

Du même auteur

The Spatial Heterogeneity of the Gut Limits Predation and Fosters Coexistence of Bacteria and Bacteriophages

Archive ouverte | Lourenço, Marta | CCSD

International audience. The ecological dynamics underlying the coexistence between antagonistic populations of bacteria and their viruses, bacteriophages (phages), in the mammalian gut microbiota remain poorly under...

Prophylactic Administration of a Bacteriophage Cocktail Is Safe and Effective in Reducing Salmonella enterica Serovar Typhimurium Burden in Vivo

Archive ouverte | Lamy-Besnier, Quentin | CCSD

International audience. Nontyphoidal Salmonella bacteria are the causative agent of salmonellosis, which accounts for the majority of foodborne illness of bacterial etiology in humans. Here, we demonstrate the safet...

The intestinal virome: lessons from animal models

Archive ouverte | Chaffringeon, Lorenzo | CCSD

International audience. Mucosal surfaces in contact with the environment host specific microbiota. The intestinal tract harbours the most abundant and diverse bacterial and viral populations interacting with each ot...

Chargement des enrichissements...