Toward Increased Reliability, Transparency, and Accessibility in Cross-linking Mass Spectrometry

Archive ouverte

Leitner, Alexander | Bonvin, Alexandre | Borchers, Christoph | Chalkley, Robert | Chamot-Rooke, Julia | Combe, Colin | Cox, Jürgen | Dong, Meng-Qiu | Fischer, Lutz | Götze, Michael | Gozzo, Fabio | Heck, Albert | Hoopmann, Michael | Huang, Lan | Ishihama, Yasushi | Jones, Andrew | Kalisman, Nir | Kohlbacher, Oliver | Mechtler, Karl | Moritz, Robert | Netz, Eugen | Novak, Petr | Petrotchenko, Evgeniy | Sali, Andrej | Scheltema, Richard | Schmidt, Carla | Schriemer, David | Sinz, Andrea | Sobott, Frank | Stengel, Florian | Thalassinos, Konstantinos | Urlaub, Henning | Viner, Rosa | Vizcaíno, Juan | Wilkins, Marc | Rappsilber, Juri

Edité par CCSD ; Elsevier (Cell Press) -

International audience. Cross-linking mass spectrometry (MS) has substantially matured as a method over the past 2 decades through parallel development in multiple labs, demonstrating its applicability to protein structure determination, conformation analysis, and mapping protein interactions in complex mixtures. Cross-linking MS has become a much-appreciated and routinely applied tool, especially in structural biology. Therefore, it is timely that the community commits to the development of methodological and reporting standards. This white paper builds on an open process comprising a number of events at community conferences since 2015 and identifies aspects of Cross-linking MS for which guidelines should be developed as part of a Cross-linking MS standards initiative.

Consulter en ligne

Suggestions

Du même auteur

π-HuB: the proteomic navigator of the human body

Archive ouverte | He, Fuchu | CCSD

International audience. The human body contains trillions of cells, classified into specific cell types, with diverse morphologies and functions. In addition, cells of the same type can assume different states withi...

MeCP2-E1 isoform is a dynamically expressed, weakly DNA-bound protein with different protein and DNA interactions compared to MeCP2-E2

Archive ouverte | Martínez de Paz, Alexia | CCSD

International audience. Background: MeCP2-a chromatin-binding protein associated with Rett syndrome-has two main isoforms, MeCP2-E1 and MeCP2-E2, differing in a few N-terminal amino acid residues. Previous studies h...

Translational repression of the Drosophila nanos mRNA involves the RNA helicase Belle and RNA coating by Me31B and Trailer hitch

Archive ouverte | Götze, Michael | CCSD

International audience. Translational repression of maternal mRNAs is an essential regulatory mechanism during early embryonic development. Repression of the Drosophila nanos mRNA, required for the formation of the ...

Chargement des enrichissements...