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Clonogrouping, a Rapid Multiplex PCR Method for Identification of Major Clones of Listeria monocytogenes

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Chenal-Francisque, Viviane | Maury, Mylène, M | Lavina, Morgane | Touchon, Marie | Leclercq, Alexandre | Lecuit, Marc | Brisse, Sylvain

Edité par CCSD ; American Society for Microbiology -

A patent application describing the clonogrouping method was submitted (V. Chenal-Francisque, M. M. Maury, M. Lavina, M. Touchon, A. Leclercq, M. Lecuit, and S. Brisse, patent application EP 15 306 154.4, 10 July 2015, European Patent Office).. International audience. Three multiplex PCR assays were developed to identify the 11 most common Listeria monocytogenes clones in clinical and food samples; 270 (95.7%) of 282 strains of serogroups IVb, IIb, IIa, and IIc were identified accurately. This novel tool is a rapid and efficient alternative to multilocus sequence typing for identification of L. monocytogenes clones.

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