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In situ photochemical crosslinking of hydrogel membrane for Guided Tissue Regeneration
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International audience. tObjective. Periodontitis is an inflammatory disease that destroys the tooth-supportingattachment apparatus. Guided tissue regeneration (GTR) is a technique based on a bar-rier membrane designed to prevent wound space colonization by gingival cells. This studyexamined a new formulation composed of two polymers that could be photochemicallycross-linked in situ into an interpenetrated polymer network (IPN) forming a hydrogel mem-brane.Methods. We synthetized and characterized silanized hydroxypropyl methylcellulose (Si-HPMC) for its cell barrier properties and methacrylated carboxymethyl chitosan (MA-CMCS)for its degradable backbone to use in IPN. Hydrogel membranes were cross-linked usingriboflavin photoinitiator and a dentistry visible light lamp. The biomaterial’s physicochem-ical and mechanical properties were determined. Hydrogel membrane degradation wasevaluated in lysozyme. Cytocompatibility was estimated by neutral red uptake. The cell bar-rier property was studied culturing human primary gingival fibroblasts or human gingivalexplants on membrane and analyzed with confocal microscopy and histological staining.Results. The IPN hydrogel membrane was obtained after 120 s of irradiation. The IPN showeda synergistic increase in Young moduli compared with the single networks. The CMCSaddition in IPN allows a progressive weight loss compared to each polymer network. Cyto-compatibility was confirmed by neutral red assay. Human cell invasion was prevented byhydrogel membranes and histological sections revealed that the biomaterial exhibited abarrier effect in contact with soft gingival tissue.
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