0 avis
cAMP and IP3 signaling pathways in HEK293 cells transfected with canine olfactory receptor genes.
Archive ouverte
Edité par CCSD ; Oxford University Press (OUP) -
Symposium Issue: Fifth International Conference on Advances in Canine and Feline Genomics and Inherited Diseases, Baltimore, Maryland, 22-25 September 2010. Corrigendum The overweight mixed breed cat photo that appeared as part of the cover for the Symposium issue: Fifth International Conference on Advances in Canine and Feline Genomics and Inherited Diseases, Baltimore, Maryland, 22-25 September 2010 (Issue 102-Supplement 1), was incorrectly identified as mixed breed and attributed to Bianca Haase. The photograph should have been credited to Katherine Parker, and the correct caption should have read as follows: The cover. This special issue of the Journal of Heredity presents the proceedings, of the 5th International Conference on Advances in Canine and Feline Genomics and Inherited Diseases. Photos are of study subjects for several of the research articles in this issue. Clockwise from left: Alaskan sled dogs (photo by Heather Huston), Maine coon cat (photo by Mary Lassaline), West Highland white terrier pups (photo by Joana Barros Roque), overweight Abyssinian cat (photo by Katherine Parker), Pug dogs (photo by Anne Yarbrough Photography) and Shetland sheepdogs (photo by Dayna Dreger). The Editorial Office wish to apologise for this error.. International audience. Olfactory receptors (ORs) expressed at the cell surface of olfactory sensory neurons lining the olfactory epithelium are the first actors of events leading to odor perception and recognition. As for other mammalian ORs, few dog OR have been deorphanized, mainly because of the absence of good methodology and the difficulties encountered to express ORs at the cell surface. Within this work, our aim was 1) to deorphanize a large subset of dog OR and 2) to compare the implication of the 2 main pathways, namely the cAMP and inositol 1,4,5-triphosphate (IP3) pathways, in the transduction of the olfactory message. For this, we used 2 independent tests to assess the importance of each of these 2 pathways and analyzed the responses of 47 canine family 6 ORs to a number of aliphatic compounds. We found these ORs globally capable of inducing intracellular calcium elevation through the IP3 pathway as confirmed by the use of specific inhibitors and/or a cAMP increase in response to aldehyde exposure. We showed that the implication of the cAMP or/and IP3 pathway was dependent upon the ligand-receptor combination rather than on one or the other partner. Finally, by exposing OR-expressing cells to the 21 possible pairs of C6-C12 aliphatic aldehydes, we confirmed that some odorant pairs may have an inhibitory or additive effect. Altogether, these results reinforce the notion that odorant receptor subfamilies may constitute functional units and call for a more systematic use of 2 complementary tests interrogating the cAMP and IP3 pathways when deorphanizing ORs.
Consulter en ligne
Suggestions
Du même auteur
