Effects of bone marrow-derived cells on monocrotaline- and hypoxia-induced pulmonary hypertension in mice.

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Raoul, William | Wagner-Ballon, Orianne | Saber, Guitanouch | Hulin, Anne | Marcos, Elisabeth | Giraudier, Stéphane | Vainchenker, William | Adnot, Serge | Eddahibi, Saadia | Maitre, Bernard

Edité par CCSD ; BioMed Central -

BACKGROUND: Bone marrow -derived cells (BMDCs) can either limit or contribute to the process of pulmonary vascular remodeling. Whether the difference in their effects depends on the mechanism of pulmonary hypertension (PH) remains unknown. OBJECTIVES: We investigated the effect of BMDCs on PH induced in mice by either monocrotaline or exposure to chronic hypoxia. METHODS: Intravenous administration of the active monocrotaline metabolite (monocrotaline pyrrole, MCTp) to C57BL/6 mice induced PH within 15 days, due to remodeling of small distal vessels. Three days after the MCTp injection, the mice were injected with BMDCs harvested from femurs and tibias of donor mice treated with 5-fluorouracil (3.5 mg IP/animal) to deplete mature cells and to allow proliferation of progenitor cells. RESULTS: BMDCs significantly attenuated PH as assessed by reductions in right ventricular systolic pressure (20 +/- 1 mmHg vs. 27 +/- 1 mmHg, P

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