Functionalization of nano-emulsions with an amino-silica shell at the oil–water interface

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Attia, Mohamed Fathy | Anton, Nicolas | Bouchaala, Redouane | Didier, Pascal | Arntz, Youri | Messaddeq, Nadia | Klymchenko, Andrey, S | Mély, Yves | Vandamme, Thierry F.

Edité par CCSD ; Royal Society of Chemistry -

International audience. A new and simple method of modify and functionalize the liquid/liquid interface of nano-emulsion droplets. , Nano-emulsions are very promising nano-carriers with high potential for loading lipophilic drugs. However, the surface of oil nano-droplets is a dynamic oil/water interface stabilized by surfactants, and its chemical modification to graft ligands is highly challenging. In this study we developed a new protocol for modification of the nano-droplets surface through a silica shell terminated by amine functions. It enabled preparation of nanocapsules of 65, 85 and 120 nm diameters with a surface coverage of ca. 2 amino groups per nm 2 . The nanocapsule surface was then functionalized (41% efficiency) by a model fluorescent ligand (coumarin blue) with a carboxylic function. The evidence for the successful grafting was provided by spectrofluorometry, Förster resonance energy transfer, atomic force microscopy coupled with fluorescence imaging and fluorescence correlation spectroscopy. This simple protocol for surface functionalization of the liquid/liquid interface of lipid droplets may constitute a real advance regarding potential applications that need efficient decoration of droplets with ligands.

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