0 avis
Unravelling genomic drivers of speciation in Musa through genome assemblies of wild banana ancestors
Archive ouverte
Edité par CCSD ; Nature Publishing Group -
Data availability: The Illumina, ONT, PACBIO HiFi, Bionano Genomics, Hi-C data, assemblies and annotations generated in this study have been deposited in the European Nucleotide Archive under project PRJEB72282. The genome assemblies and gene and TE annotations are available at Banana Genome Hub [http://banana-genome-hub.southgreen.fr]. GBSS and ADH alignments, EDTA repeat database and LTR repeat database produced are available on the Banana Genome Hub [http://banana-genome-hub.southgreen.fr] in the download section and on CIRAD dataverse [https://doi.org/10.18167/DVN1/6TLMD3]. The GBS data are available in the Short Read Archive under the following projects PRJNA1078411, PRJNA1182927 and PRJNA667853. Germplasm is available at the CIRAD-INRAE Biological Resource Centre for Tropical Plants (CRB-PT) in the West Indies (Guadeloupe, France) for accessions PT-BA-00024 (M. a. ssp. banksii); PT-BA-00228 for M. textilis; PT-BA-00182 for M. a. ssp. zebrina; PT-BA-00051 for M. a. ssp. burmannicoides and PT-BA-00304 for the diploid cultivated Pisang Madu. Germplasm of M. schizocarpa is available at the Bioversity International Transit Center in Leuven (Belgium) under ITC0926 accession number. Publicly available sequencing data (PRJEB58004 [https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJEB58004] and PRJEB26661) were also used in this study. Source data are provided with this paper.Code availability: Custom code has been added to vcfHunter toolbox that is available at Github [https://github.com/SouthGreenPlatform/VcfHunter].. International audience. Hybridization between wild Musa species and subspecies from Southeast Asia is at the origin of cultivated bananas. The genomes of these cultivars are complex mosaics involving nine genetic groups, including two previously unknown contributors. This study provides continuous genome assemblies for six wild genetic groups, one of which represents one of the unknown ancestor, identified as M. acuminata ssp. halabanensis. The second unknown ancestor partially present in a seventh assembly appears related to M. a. ssp. zebrina. These assemblies provide key resources for banana genetics and for improving cultivar assemblies, including that of the emblematic triploid Cavendish. Comparative and phylogenetic analyses reveal an ongoing speciation process within Musa, characterised by large chromosome rearrangements and centromere differentiation through the integration of different types of repeated sequences, including rDNA tandem repeats. This speciation process may have been favoured by reproductive isolation related to the particular context of climate and land connectivity fluctuations in the Southeast Asian region.
Consulter en ligne
Suggestions
Du même auteur
