A protocol for inducing long-term plasticity of intrinsic neuronal excitability in neurons of the dorsal lateral geniculate nucleus

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Russier, Michaël | Duménieu, Maël | Wakade, Anushka | Incontro, Salvatore | Fronzaroli- Molinieres, Laure | Debanne, Dominique

Edité par CCSD ; JoVE -

International audience. The dorsal lateral geniculate nucleus (dLGN) has long been held to act as a basic relay forvisual information travelling from the retina to cortical areas, but recent findings suggest largelyunderestimated functional plasticity of dLGN principal cells. However, the cellular mechanismssupporting these changes have not been fully explored. We report here a protocol to inducelong-term potentiation of intrinsic neuronal excitability (LTP-IE) in dorsal dLGN relay cells fromacute brain slices of young rats. Intrinsic plasticity is generally induced in parallel with synapticplasticity. However, in dLGN neurons, LTP-IE is reliably induced by spiking activity at afrequency of 40 Hz during 10 minutes. LTP-IE in dLGN relay neurons is long-lasting as it can befollowed up to 40 min after the induction protocol. In conclusion, our results provide the first2evidence for induction of intrinsic plasticity in dLGN relay cells, thus further pointing the role ofthalamic neurons in activity-dependent visual plasticity.

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