TRPP2-dependent Ca2+ signaling in dorso-lateral mesoderm is required for kidney field establishment in Xenopus

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Futel, Mélinée | Leclerc, Catherine | Le Bouffant, Ronan | Buisson, Isabelle | Néant, Isabelle | Umbhauer, Muriel | Moreau, Marc | Riou, Jean-François

Edité par CCSD ; Company of Biologists -

International audience. In Xenopus embryos kidney field (KF) specification is dependent on retinoic acid (RA) and coincides with a dramatic increase of Ca2+ transients, but the role of Ca2+ signaling in the KF is unknown. Here, we identify TRPP2, a TRP superfamily member of channel encoded by the pkd2 gene, as a central component of Ca2+ signaling in the KF. TRPP2 is strongly expressed at the plasma membrane where it may regulate extracellular Ca2+ entry. Knockdown of pkd2 in the KF results in the down-regulation of pax8, but not of the other KF genes lhx1, osr1 and 2. We further show that inhibition of Ca2+ signaling by an inducible Ca2+ chelator also causes pax8 inhibition, and that pkd2 knockdown results in a severe inhibition of Ca2+ transients in KF explants. Finally we show that disruption of RA results both in an inhibition of intracellular Ca2+ signaling and of TRPP2 incorporation into the plasma membrane of KF cells. We propose that TRPP2-dependent Ca2+ signaling is a key component of pax8 regulation in the KF downstream of a RA non-transcriptional control of TRPP2.

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