Timber tracking of Jacaranda copaia from the Amazon Forest using DNA fingerprinting

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Capo, Lorena, F. M. | Degen, Bernd | Blanc-Jolivet, Celine | Tysklind, Niklas | Cavers, Stephen | Mader, Malte | Meyer-Sand, Barbara, R.V. | Paredes-Villanueva, Kathelyn | Honorio Coronado, Euridice N. | García-Dávila, Carmen | Troispoux, Valérie | Delcamp, Adline | Sebbenn, Alexandre

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We investigated the utility of nuclear and cytoplasmic single nucleotide polymorphism (SNP) markers for timber tracking of the intensively logged and commercialized Amazonian tree Jacaranda copaia. Eight hundred thir-ty-two trees were sampled (cambium or leaves) from 38 sampling sites in Bolivia, Brazil, French Guiana, and Peru. A total of 128 SNP markers (113 nuclear, 11 chloroplastic, and 4 mitochondrial) were used for genotyping the samples. Bayesian cluster analyses were carried out to group individuals into homogeneous genetic groups for self-assignment tests of groups of indi-viduals or of individuals to their population of origin. Cluster analysis based on all SNP markers detected seven main genetic groups. Genetic differentia-tion was high among populations (0.484) and among genetic groups (0.415), and populations showed a strong isolation-by-distance pattern. Self-assignment test of groups of individual for all loci was able to determine the population origin of all samples (accuracy= 100%). Self-assignment tests of individuals was able to assigin the origin of 94.5–100% of individuals (accuracy: 91.7–100%). Our results show that the use of 128 SNP markers is suitable to correctly determine the origin of J. copaia timber and they should be considered a useful tool for customs and local and international police.

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