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Dynamic Hybrid Model for Nanobody-based Antivenom Production (scorpion antivenon) with E. coli CH10-12 and E. coli NbF12-10. Modélisation hybride et dynamique de la production d'un nanobody anti-venin (de scorpion) dans E. coli CH10-12 et E. coli NbF12-10
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The 34th European Symposium on Computer Aided Process Engineering, 15th International Symposium on Process Systems Engineering: ESCAPE-34/PSE2024, Volume 53 provides the latest information to come out of the International Symposium on Process Systems Engineering joint event. It is a valuable resource for chemical engineers, chemical process engineers, researchers in industry and academia, students, and consultants for chemical industries.. International audience. Immunotherapy is a specific treatment for scorpion stings, with antibody fragments being used to neutralize scorpion neurotoxins. Nanobodies (VHH), fragments of camelid antibodies, were successfully produced intracellularly in Escherichia coli WK6 in fedbatch cultures. Their production was further enhanced by dynamic modelling. Two dynamic modelling approaches to describe nanobody CH12-10 production as a function of the induction temperature are proposed in this work. The first one is a kinetic model and the second is a hybrid approach, coupling a mass balance with support vector machine (SVM). Both models were calibrated and validated with independent data sets. Results reveal that the hybrid model procures better predictions than the kinetic model. Finally, the hybrid model was improved by retraining the SVM Model, resulting in a Normed Root Mean Square Error (NRMSE) values between 0.1148 and 0.8523.
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