Basolateral Localization and Transcytosis of Gonadotropin and Thyrotropin Receptors Expressed in Madin-Darby Canine Kidney Cells

Archive ouverte

Beau, Isabelle | Misrahi, Micheline | Gross, Babette | Vannier, Brigitte | Loosfelt, Hugues | Hai, Mai Thu Vu | Pichon, Christophe | Milgrom, Edwin

Edité par CCSD ; American Society for Biochemistry and Molecular Biology -

International audience. The thyrotropin (TSH) and follicle stimulating hormone (FSH) receptors are present mainly on the basolateral cell surface in the thyroid gland and in Sertoli cells, whereas in ovarian and in testicular cells, the luteinizing hormone (LH) receptors are distributed throughout the cell surface, When expressed in Madin-Darby canine kidney (MDCK) cells, all three receptors accumulated at the basolateral cell surface showing that they carry the corresponding targeting signals, The receptors were directly delivered to the basolateral surface of the MDCK cells, A minor fraction of the gonadotropin receptors but not of TSH receptors was secondarily targeted to the apical surface through transcytosis, The mechanisms of basolateral targeting and transcytosis were analyzed using the FSH receptor as a model, Both were insensitive to brefeldin A and pertussis toxin, G(s) activation by AlF4- and cholera toxin provoked a marked enhancement of FSH receptor transcytosis, The population of G(s) proteins involved in this mechanism was different from that involved in signal transduction since neither FSH nor forskolin mimicked the effects of AlF4- and cholera toxin, G(s) activation provoked a similar effect on LH receptor distribution in MDCK cells, whereas it did not modify the compartmentalization of the TSH receptor, Hormone-specific transcytosis was observed in MDCK cells expressing the gonadotropin (FSH and LH) receptors and was increased after cholera toxin administration.

• Description
• Sujet/Public
• Outil
• Outil d'anticipation
• Mémoire et thèse
• Gestion