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Implementation of CDH in LPMO-Containing Minimal Cellulase Cocktails to Tune Bioproduct Production from Lignocellulosic Biomass. Mise en œuvre de CDH dans les cocktails de cellulase minimale contenant des LPMO permet d'ajuster la production de bioproduits à partir de biomasse lignocellulosique
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Edité par CCSD ; American Chemical Society -
International audience. Plant biomass can be converted to bioproducts using fungal enzymatic cocktails that have been improved upon implementation of lytic polysaccharide monooxygenases (LPMOs), known to boost cellulases. However, due to the complexity of their oxidative catalysis, it is still difficult to properly control and sustain LPMOs activity. Here, we investigated whether the implementation of the natural enzymatic partner of LPMOs, i.e. cellobiose dehydrogenase (CDH), could be a promising solution. To this end, we reconstituted a minimal cocktail using the main cellulases from Trichoderma reesei, and evaluated the impact of the addition of two LPMOs and one CDH on the conversion of wheat straw, miscanthus, pine and poplar. Surprisingly, while the addition of LPMOs or LPMO/CDH showed little to no increase in glucose equivalent yields, the sole addition of CDH caused an increase of up to 27% (on miscanthus). Interestingly, CDH supplementation allowed to tune the stream of degradation products towards cellobionic acid while maintaining equivalent or increasing the overall conversion yields, in a biomass-dependent manner. Our study shows that extracting reasonable amounts of high value-added oxidized sugars from industrial biomass is feasible, opening thereby new perspectives for the use of CDH in industry.
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