Immunoprecipitation Methods to Isolate Messenger Ribonucleoprotein Complexes (mRNP)

0 avis

Immunoprecipitation Methods to Isolate Messenger Ribonucleoprotein Complexes (mRNP)

Archive ouverte

Edité par CCSD ; Springer Nature Switzerland -

International audience. Throughout their life cycle, messenger RNAs (mRNAs) associate with proteins to form ribonucleoproteins (mRNPs). Each mRNA is part of multiple successive mRNP complexes that participate in their biogenesis, cellular localization, translation and decay. The dynamic composition of mRNP complexes and their structural remodelling play crucial roles in the control of gene expression. Studying the endogenous composition of different mRNP complexes is a major challenge. In this chapter, we describe the variety of protein-centric immunoprecipitation methods available for the identification of mRNP complexes and the requirements for their experimental settings.

Langue: en

ISBN: : 978-3-031-52195-9

Indexation

Consulter en ligne

Consulter le document

Suggestions

Du même auteur

Tracking the m 7 G-cap during translation initiation by crosslinking methods

Archive ouverte | Gross, Lauriane | CCSD

International audience

Purification of In Vivo or In Vitro-Assembled RNA-Protein Complexes by RNA Centric Methods. Purification de complexes ARN-protéine assemblés in vivo ou in vitro par des méthodes centrées sur l'ARN

Archive ouverte | Janvier, Aurélie | CCSD

International audience. Throughout their entire life cycle, RNAs are associated with RNA-binding proteins (RBPs), forming ribonucleoprotein (RNP) complexes with highly dynamic compositions and very diverse functions...

eIF3 interacts with histone H4 messenger RNA to regulate its translation

Archive ouverte | Hayek, Hassan | CCSD

International audience. This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the def...

Chargement des enrichissements...