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G-quadruplexes in Haloferax volcanii

Archive ouverte | Aktary, Zackie | CCSD

International audience. The archaeal domain is a taxonomically rich component of microbial communities that inhabit a wide range of habitats on Earth, including the human body. Phylogenomic analyses have indicated t...

BrdU Incorporation and Labeling of Nascent DNA to Investigate Archaeal Replication Using Super-Resolution Imaging

Archive ouverte | Lestini, Roxane | CCSD

International audience. The labeling and specific detection of nascent DNA by the incorporation of thymidine analogs provide crucial information about DNA replication dynamics without requiring the intracellular exp...

The Escherichia coli UvrD helicase is essential for Tus removal during recombination-dependent replication restart from Ter sites.

Archive ouverte | Bidnenko, Vladimir | CCSD

Blocking replication forks in the Escherichia coli chromosome by ectopic Ter sites renders the RecBCD pathway of homologous recombination and SOS induction essential for viability. In this work, we show that the E. coli helicase I...

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