Measuring the transcriptome-wide effects of aging on murine adipocytes using RNAseq

0 avis

Measuring the transcriptome-wide effects of aging on murine adipocytes using RNAseq

Archive ouverte

Edité par CCSD ; Elsevier -

International audience. Adipose tissue plays a central role in age-related diseases. While RNAseq protocols exist for many tissues, few data have been generated with this technology to explore gene expression in adipocytes, particularly during aging. Here, we present a protocol to analyze the transcriptional changes that occur in adipose tissue during normal and accelerated aging in mouse models. We describe steps for genotyping, diet control, euthanasia, and dissection. We then detail RNA purification and genome-wide data generation and analysis. For complete details on the use and execution of this protocol, please refer to De Cauwer et al. (2022) iScience. Sep 16;25(10):105149.

Langue: en

Consulter en ligne

Consulter le document

Suggestions

Du même auteur

Implication of Dicer1 gene in ageing and age-related pathologies in mice. Implication du gène Dicer1 dans le vieillissement et ses pathologies chez la souris

Archive ouverte | de Cauwer, Aurore | CCSD

Despite a 30-year gain in life expectancy over the last century in developed countries, reaching old age remains associated with the development of chronic and/or disabling pathologies. Defining the actors of aging is therefore a ...

DICER1: A Key Player in Rheumatoid Arthritis, at the Crossroads of Cellular Stress, Innate Immunity, and Chronic Inflammation in Aging

Archive ouverte | de Cauwer, Aurore | CCSD

International audience. FiGuRe 1 | Canonical and non-canonical functions of DICER1 in the nucleus and the cytosol. The canonical function of DICER1 leads to microRNA maturation in the cytoplasm. Others functions are...

Dicer1 deficient mice exhibit premature aging and metabolic perturbations in adipocytes

Archive ouverte | de Cauwer, Aurore | CCSD

Chargement des enrichissements...

Syracuse